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B-hCD30 mice
Strain Name C57BL/6-Cd30tm1(CD30)Bcgen/Bcgen Common Name  B-hCD30 mice
Background C57BL/6 Catalog number 110888
Aliases 
TNFRSF8, Ki-1, D1S166E
Protein expression analysis in T cells

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Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice. 

Protein expression analysis in NK cells

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Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice. 

mRNA expression analysis

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Strain specific analysis of CD30 mRNA expression in wild-type C57BL/6 mice and B-hCD30 mice by RT-PCR. Mouse Cd30 mRNA was detectable only in splenocytes of wild-type C57BL/6 mice. Human CD30 mRNA was detectable only in homozygous B-hCD30 mice but not in wild-type mice.