Strain specific CD3E expression analysis in homozygous B-hCD3E/hB7-H3 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H3 mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+).

Strain specific analysis of B7-H3 expression in wild type (WT) mice and B-hCD3E/hB7-H3 mice by RT-PCR and WB. Lung were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H3 mice (H/H; H/H), and analyzed by RT-PCR and western blot with anti-human B7-H3 antibody. Mouse B7-h3 mRNA was detectable only in lung of WT mice (+/+). Human B7-H3 mRNA was detectable only in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing. Human B7-H3 was exclusively detectable in homozygous B-hCD3E/hB7-H3 mice (H/H; H/H).