Strain specific analysis of B7-H4 gene expression in wild type (WT) mice and B-hCD3E/hB7-H4 mice by RT-PCR. Mouse B7-h4 mRNA was detectable only in lung of WT mice (+/+). Human B7-H4 mRNA was detectable only in homozygous B-hCD3E/hB7-H4 mice (H/H) but not in WT mice (+/+). 

Strain specific CD3E/hB7-H4 expression analysis in homozygous B-hCD3E/hB7-H4 mice by western blot. Lung, uterus, heart, kidney and breast were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H4 mice (H/H), and analyzed by western blot with anti-B7-H4 antibody. B7-H4 was detectable in WT mice (+/+), homozygous B-hCD3E/hB7-H4 mice (H/H) and human B7-H4 positive cell line SK-BR-3 due to the cross-reactivity of antibodies. 

Strain specific CD3E expression analysis in homozygous B-hCD3E/hB7-H4 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hB7-H4 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hB7-H4 mice (H/H) but not in WT mice (+/+).