| Strain Name | C57BL/6-Cd3etm2(CD3E)Bcgen Cd19tm4(CD19)Bcgen Ms4a1tm2(MS4A1)Bcgen/Bcgen | Common Name | B-hCD3E/hCD19/hCD20 mice |
| Background | C57BL/6 | Catalog number |
113171 |
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Related Genes |
CD3epsilon, IMD18, T3E, TCRE B4, CVID3 B1, Bp35, CD20, CVID5, FMC7, LEU-16, S7 |
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- CD3 consists of four protein chains (CD3E, CD3D, CD3G and CD3Z), which are important biological markers on the T cell membrane. CD3 can form a TCR/CD3 complex with the T cell receptor, participating in the regulation of T cell antigen recognition, signal transduction and T cell development. CD19 is a biomarker for normal and neoplastic B cells, as well as follicular dendritic cells. CD19 is critically involved in establishing intrinsic B cell signaling thresholds through modulating both B cell receptor-dependent and independent signaling. CD20 is a marker of B cells, and it begins to express in Pre-B cells and loses expression after differentiating into plasma cells. CD20 is expressed on the surface of normal and about 95% malignant B lymphocytes, but not expressed in hematopoietic stem cells, plasma cells, or other normal tissues. CD20 is regarded as an ideal target for the treatment of B-cell lymphoma and autoimmune diseases
- B-hCD3E/hCD19/hCD20 mice were obtained by mating B-hCD3E mice(110008), B-hCD19 mice(112657) and B-hCD20 mice(111231). In this mice, the exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7. The chimeric CD19 coding sequence containing the extracellular region of human CD19 gene is inserted into mouse Cd19 gene locus site. The exons 2-7 of mouse Cd20 gene that encode the whole molecule (ATG to STOP codon) are replaced by human counterparts in B-hCD3E/hCD19/hCD20 mice.
- Human CD3E can be detected on T cells from spleen of homozygous B-hCD3E/hCD19/hCD20 mice, but not in wild-type mice. Human CD19 and CD20 can be detected on B cells from spleen of homozygous B-hCD3E/hCD19/hCD20 mice. Following humanization of CD3E, CD19, and CD20, the percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in B-hCD3E/hCD19/hCD20 mice were similar to those wild-type mice.
- This product is used for the pharmacological and safety evaluation of therapeutic drugs, such as anti-CD19 or anti-CD20 antibodies, bi or tri-specific antibodies, CAR-T cell therapies, and other drugs. Disease areas include oncology and autoimmune diseases.
Strain specific CD3E expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3E/hCD19/hCD20 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and homozygous B-hCD3E/hCD19/hCD20 mice (female, 8-week-old, n=1). Protein expression was analyzed with anti-mouse CD3E antibody (Biolegend, 100312) and anti-human CD3E antibody (BD Horizon™, 562426) by flow cytometry. Mouse CD3E was only detectable in wild-type C57BL/6JNifdc mice. Human CD3E was exclusively detectable in homozygous B-hCD3E/hCD19/hCD20 mice, but not in wild-type C57BL/6JNifdc mice.
Protein expression analysis-CD19
Strain specific CD19 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3E/hCD19/hCD20 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and homozygous B-hCD3E/hCD19/hCD20 mice (female, 8-week-old, n=1). Protein expression was analyzed with anti-mouse CD19 antibody (Biolegend, 115507) and anti-human CD19 antibody (Biolegend, 302234) by flow cytometry. Mouse CD19 was only detectable in wild-type C57BL/6JNifdc mice. Human CD19 was exclusively detectable in homozygous B-hCD3E/hCD19/hCD20 mice, but not in wild-type C57BL/6JNifdc mice.
Frequency of leukocyte subpopulations in lymph nodes by flow cytometry. Lymph nodes cells were isolated from wild-type C57BL/6JNifdc mice (female, n=3, 8-week-old) and homozygous B-hCD3E/hCD19/hCD20 mice (female, n=3, 8-week-old). A. Flow cytometry analysis of the lymph nodes cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, and NK cells in B-hCD3E/hCD19/hCD20 mice were similar to those wild-type mice. Values are expressed as mean ± SEM.
