| Strain Name |
C57BL/6-Cd3etm2(CD3E)Bcgen Il2ratm1(IL2RA)Bcgen/Bcgen
|
Common Name | B-hCD3E/hIL2RA mice |
| Background | C57BL/6 | Catalog number |
121666 |
|
Related Genes |
CD3E, IMD18, T3E, TCRE; IL2RA,CD3e molecule; IL2RA, CD25, IDDM10, IL2R, IMD41, TCGFR, p55, interleukin 2 receptor subunit alpha | ||
|
NCBI Gene ID |
12501,16184 |
||
Protein expression analysis in T cells
Strain specific CD3E expression analysis in homozygous B-hCD3E/hIL2RA mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hIL2RA mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hIL2RA mice (H/H;H/H) but not in WT mice (+/+).
Protein expression analysis in T cells
Strain specific IL2RA expression analysis in homozygous B-hCD3E/hIL2RA mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hIL2RA mice (H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RA antibody. Mouse IL2RA was detectable in WT mice (+/+). Human IL2RA was exclusively detectable in homozygous B-hCD3E/hIL2RA mice (H/H;H/H) but not in WT mice (+/+).
