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Strain Name
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C57BL/6-Cd47tm1(CD47)Bcgen/Bcgen
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Common Name
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B-hCD47 mice
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Background
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C57BL/6
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Catalog number
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110010
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Aliases
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Cd47 (CD47 antigen)
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NCBI Gene ID
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16423
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Protein Expression Analysis
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD47 mice were analyzed by flow cytometry. Mouse CD47+ T cells were detectable in the WT C57BL/6 mice, while human CD47+ T cells were detectable in the homozygous B-hCD47 mice.
Human CD47 Abs Toxicity Evaluation
Homozygous B-hCD47 mice were injected with various anti-hCD47 antibodies via i.p. injection. Body weight was measured daily. The results indicated toxicity of different antibodies. hCD47 Ab4 and hCD47 Ab6 showed high toxicity levels, as mice died. Y-axis showed average body weight ± SEM.
Human CD47 mAb toxicity evaluation
Homozygous B-hCD47 mice were i.p. injected with anti-hCD47 antibodies. Blood from each mice were collected and Blood Routine Test was conducted. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.
Toxicity evaluation of anti-human CD47 antibodies
Blood biochemical analysis of toxicity in mice injected with hCD47 antibody
Homozygous B-hCD47 mice were I.P. injected with anti-hCD47 antibodies. Blood from each mice were collected and Blood Biochemical Test was conducted. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.
In vivo efficacy of anti-human CD47 antibody
Antitumor activity of anti-human CD47 antibody in B-hCD47 mice and C57BL/6 mice. Anti-human CD47 antibody inhibited MC38-hCD47 tumor growth in B-hCD47 mice. (A) Murine colon cancer MC38-hCD47 cells (5×105) were subcutaneously implanted into homozygous B-hCD47 mice (female, 6 week-old, n=6). Mice were grouped when tumor volume reached approximately 150 mm3, at which time they were treated with two anti-human CD47 antibodies and schedules indicated in panel A, as shown in panel A, 3 mg/kg of anti-human CD47 Ab2 was efficacious in controlling tumor growth in the homozygous B-hCD47 mice.(B)Body weight. The results demonstrating that B-hCD47 mice provide a powerful preclinical model for in vivo evaluation of anti-human CD47 antibodies. Values are expressed as mean ± SEM.
