B-hCD89 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Humanizad Mouse Models

Text

B-hCD89 mice

Strain Name	C57BL/6N-Gt(ROSA)26Sor^{tm1(CD11b-FCAR)Bcgen}/Bcgen	Common Name	B-hCD89 mice
Background	C57BL/6N	Catalog number	110864
Aliase	CD89; FcalphaRI; CTB-61M7.2, FCAR, Fc fragment of IgA receptor

mRNA expression analysis

Strain specific analysis of CD89 gene expression in wild type (WT) mice and B-hCD89 mice by RT-PCR. Human CD89 mRNA was detectable only in splenocytes of homozygous B-hCD89 mice (H/H) but not WT mice (+/+).

Protein expression analysis in NK cells

Strain specific CD89 expression analysis in homozygous B-hCD89 mice by flow cytometry. Spleen, bone marrow and blood were collected from wild type (WT) mice (+/+) and homozygous B-hCD89 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD89 antibody. Human CD89 was exclusively detectable in NK cells of homozygous B-hCD89 mice (H/H).

Protein expression analysis in CD11b+ cells

Analysis of blood and urine

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Blood and urine analysis: Four 48-weeks-old male B-hCD89 mice(H/H) and C57BL/6N were selected to assess the blood and urine indicators. The results showed that the levels of creatinine (CREA) and urine protein in the urine, as well as blood urea nitrogen (UREA) and creatinine (CREA), did not show significant differences compared to wild mice.

Analysis of kidney tissue sections

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Analysis of kidney tissue sections: Four 48-week-old male B-hCD89 mice(H/H) and C57BL/6N were selected for kidney tissue section immunohistochemical staining analysis. The results showed IgA and C3 staining showed no significant differences between B-hCD89 mice(H/H) and C57BL/6N.