| Strain Name |
C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfrsf18tm1(TNFRSF18)Bcgen/Bcgen
|
Common Name | B-hCTLA4/hGITR mice |
| Background | C57BL/6 | Catalog number | 120962 |
|
Related Genes |
CTLA4 (cytotoxic T-lymphocyte-associated protein 4) TNFRSF18 (tumor necrosis factor receptor superfamily, member 18) |
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|
NCBI Gene ID |
12477,21936 | ||
mRNA expression analysis
Strain specific analysis of CTLA4 and GITR gene expression in WT and B-hCTLA/hGITR mice by RT-PCR. Mouse Ctla4 and Tnfrsf18 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CTLA4 and GITR mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Strain specific CTLA4 and GITR expression analysis in homozygous B-hCTLA4/hGITR mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCTLA4/hGITR (H/H) mice analyzed by flow cytometry with species-specific anti-CTLA4 antibody or anti-GITR antibody. Mouse CTLA4 and GITR were exclusively detectable in WT mice. Human CTLA4 and GITR were exclusively detectable in homozygous B-hCTLA4/hGITR but not WT mice.
Strain specific CTLA4 and GITR expression analysis in homozygous B-hCTLA4/hGITR mice by flow cytometry.
Splenocytes were collected from WT and homozygous B-hCTLA4/hGITR (H/H) mice stimulated with anti-CD3ε in vivo (7.5 μg/mice), and analyzed by flow cytometry with species-specific anti-CTLA4 antibody or anti-GITR antibody. Mouse CTLA4 and GITR were exclusively detectable in WT mice. Human CTLA4 and GITR were exclusively detectable in homozygous B-hCTLA4/hGITR but not WT mice.
Combination therapy of anti-human CTLA4 antibody and anti-human GITR antibody
