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B-hCTLA4/hOX40 mice
Strain Name
C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfrsf4tm1(TNFRSF4)Bcgen/Bcgen
Common Name  B-hCTLA4/hOX40 mice
Background C57BL/6 Catalog number  120531
Related Genes 
Ctla4 (cytotoxic T-lymphocyte-associated protein 4)
Tnfrsf4 
(Tumor necrosis factor receptor superfamily, member 4, also known as OX40)
NCBI Gene ID
12477,22163

Protein expression analysis




Strain specific CTLA4 and OX40 expression analysis in homozygous B-hCTLA4/hOX40 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCTLA4/hOX40 (H/H) mice stimulated with anti-CD3ε in vivo (7.5 μg/mice), and analyzed by flow cytometry with species-specific anti-CTLA4 antibody. Mouse CTLA4 and OX40 were exclusively detected in WT mice. Human CTLA4 CTLA4 and OX40 were exclusively detected in homozygous B-hCTLA4/hOX40 but not WT mice.


Combination therapy of CTLA4 mAb (ipilimumab) and OX40 mAb


from clipboard

Antitumor activity of anti-hOX40 antibody combined with anti-hCTLA4 antibody ipilimumab in B-hCTLA4/hOX40 mice. (A) Anti-hOX40 antibody combined with anti-hCTLA4 antibodies ipilimumab inhibited MC38 tumor growth in B-hCTLA4/hOX40 mice. Murine colon cancer MC38 cells (5×105) were subcutaneously implanted into homozygous B-hCTLA4/hOX40 mice (female, 5-8 week-old, n=8). Mice were grouped when tumor volume reached approximately 150±50 mm3, at which time they were treated with anti-hOX40 antibody combined with anti- hCTLA4 antibody ipilimumab with doses and schedules indicated in panel (B) Body weight changes during treatment. As shown in panel A, combination of anti-hOX40 and anti-hCTLA4 antibody shows more inhibitory effects than individual groups, demonstrating that the B-hCTLA4/hOX40 mice provide a powerful preclinical model for in vivo evaluating combination therapy efficacy of hOX40 antibodies and h B-hCTLA4 antibodies. Values are expressed as mean ± SEM.