Protein expression analysis

Strain specific TIM3 and CTLA4 expression analysis in homozygous B-hCTLA4/hTIM3 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCTLA4/hTIM3 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TIM3 and anti-CTLA4 antibody. Mouse TIM3 and CTLA4 were detectable in WT mice. Human TIM3 amd CTLA4 were exclusively detectable in homozygous B-hCTLA4/hTIM3 but not WT mice.