Strain specific analysis of DDR1 gene expression in wild-type C57BL/6 mice and B-hDDR1 mice by RT-PCR. Mouse Ddr1 mRNA was detectable only in brain of wild-type C57BL/6 mice (+/+). Human DDR1 mRNA was detectable only in homozygous B-hDDR1 mice (H/H), but not in wild-type mice. 

Strain specific DDR1 expression analysis in homozygous B-hDDR1 mice by western blot. Brain collected from wild-type C57BL/6 mice (+/+) and homozygous B-hDDR1 mice (H/H), and analyzed by western blot with DDR1 antibodies. Human DDR1 was exclusively detectable in homozygous B-hDDR1 but not in wild-type mice. Mouse DDR1 was detectable in wild-type mice. The DDR1 antibody is a human-specific antibody and the other is a human-mouse cross-recognition antibody.