Strain specific analysis of FGL1 gene expression in wild-type C57BL/6 mice and B-hFGL1/hLAG3 plus mice by RT-PCR. Mouse Fgl1 mRNA was detectable only in liver of wild-type C57BL/6 mice (+/+). Human FGL1 mRNA was detectable only in homozygous B-hFGL1/hLAG3 plus mice (H/H; H/H), but not in wild-type mice. 

Strain specific FGL1 expression analysis in homozygous B-hFGL1/hLAG3 plus mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFGL1/hLAG3 plus mice (H/H;H/H), and analyzed by ELISA with species-specific FGL1 ELISA kit. Human FGL1 was exclusively detectable in homozygous B-hFGL1/hLAG3 plus mice (H/H;H/H) but not wild-type mice.

Strain specific LAG3 expression analysis in homozygous B-hFGL1/hLAG3 plus mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hFGL1/hLAG3 plus mice (H/H; H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-LAG3 antibody. Mouse LAG3 was detectable in C57BL/6 (+/+) . Human LAG3 was exclusively detectable in homozygous B-hFGL1/hLAG3 plus mice (H/H; H/H) but not WT mice.