Strain specific analysis of FGL2 gene expression in wild-type mice and B-hFGL2 mice by RT-PCR. Mouse Fgl2 mRNA was detectable in peritoneal exudative macrophages of wild-type mice (+/+). Human FGL2 mRNA was detectable only in homozygous B-hFGL2 mice (H/H) but not in wild-type mice.

Strain specific FGL2 expression analysis in homozygous B-hFGL2 mice by western blot. Peritoneal exudative macrophages and splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFGL2 mice (H/H) sitimulated by mIFNγ 20ng/ml in vitro for 24h, and analyzed by western blot with anti-FGL2 antibody. FGL2 was detectable in wild type mice and homozygous B-hFGL2 mice, as the antibody is crossly reactive with FGL2 in human and mice.