| Strain Name | C57BL/6N-Icam1tm1(ICAM1)Bcgen/Bcgen | Common Name | B-hICAM1 mice |
| Background | C57BL/6N | Catalog number | 111242 |
| Aliases |
ICAM1(BB2, CD54, P3.58) |
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Targeting strategy
Gene targeting strategy for B-hICAM1 mice. The exons 2-7 of mouse Icam1 that encode the full-length protein, not including signal peptides were replaced by the exons 2-7 of human ICAM1 gene in B-hICAM1 mice.
Protein expression analysis of ICAM1 in B cells and T cells
Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry. T cells and B cells from the spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibodies. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.
Protein expression analysis of ICAM1 in NK cells and dendritic cells
Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry. NK cells, dendritic cells from the spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibodies. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.
Protein expression analysis of ICAM1 in granulocytes, monocytes, and macrophages
Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry. Granulocytes, monocytes, and macrophages from the spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibodies. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.
