top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hOX40 mice
Strain Name
C57BL/6-Tnfrsf4tm1(TNFRSF4)Bcgen/Bcgen
Common Name  B-hOX40 mice
Background C57BL/6 Catalog number  110014
Related Genes 
TNFRSF4 (tumor necrosis factor receptor superfamily, member 4)
NCBI Gene ID
22163

mRNA expression analysis


from clipboard


Strain specific analysis of OX40 gene expression in WT and hOX40 mice by RT-PCR. Mouse Ox40 mRNA was detected in splenocytes of wild-type (+/+) mice. Human OX40 mRNA was detected only in homozygous B-hOX40 (H/H) mice .


Protein expression analysis

from clipboard


Strain specific OX40 expression analysis in homozygous B-hOX40 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hOX40 (H/H) mice stimulated with anti-CD3ε in vivo (7.5 μg/mice), and analyzed by flow cytometry with species-specific anti-OX40 antibody. Mouse OX40 was exclusively detected in WT mice. Human OX40 was exclusively detected in homozygous B-hOX40 but not WT mice.


In vivo efficacy of anti-human OX40 antibody

from clipboard

Antitumor activity of anti-human OX40 antibody in B-hOX40 mice. (A) Anti-human OX40 antibody inhibited MC38 tumor growth in B-hOX40 mice. Murine colon cancer MC38 cells (5ⅹ105) were subcutaneously implanted into homozygous B-hOX40 mice (male, 4-6 week-old, n=5). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with anti-human OX40 antibody and schedules indicated in panel (B) Body weight changes during treatment. As shown in panel A, anti-human OX40 antibody was efficacious in controlling tumor growth in B-hOX40 mice, demonstrating that the B-hOX40 mice provide a powerful preclinical model for in vivo evaluation of anti-human OX40 antibodies. Values are expressed as mean ± SEM


In vivo efficacy of MOXR0916 (in house)

from clipboard


Antitumor activity of anti-human OX40 antibody in B-hOX40 mice. (A) Anti-human OX40 antibody inhibited MC38 tumor growth in B-hOX40 mice. Murine colon cancer MC38 cells (5ⅹ105) were subcutaneously implanted into homozygous B-hOX40 mice (female, 4-6 week-old, n=6). Mice were grouped when tumor volume reached approximately 150±50 mm3, at which time they were treated with anti-human OX40 antibody with different doses and schedules indicated in panel (B) Body weight changes during treatment. As shown in panel A, anti-human OX40 antibody MOXR0916(in house) was efficacious in controlling tumor growth in B-hOX40 mice, demonstrating that the B-hOX40 mice provide a powerful preclinical model for in vivo evaluation of anti-human OX40 antibodies. Values are expressed as mean ± SEM