Strain specific PD-1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Mouse PD-1 was detectable in WT mice (+/+). Human PD-1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).

Strain specific CD3E expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).

Strain specific PD-L1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was detectable in WT mice (+/+). Human PD-L1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).

Strain specific PD-L1 expression analysis in homozygous B-hPD-1/hPD-L1/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was detectable in WT mice (+/+). Human PD-L1 was exclusively detectable in homozygous B-hPD-1/hPD-L1/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).