| Strain Name |
C57BL/6N-Agertm2(AGER) Bcgen/Bcgen
|
Common Name | B-hRAGE mice plus |
| Background | C57BL/6N | Catalog Number | 113157 |
|
Aliases |
RAGE, SCARJ1, sRAGE |
||
|
NCBI Gene ID |
177 | ||
- RAGE is expressed in more than 300 cells in addition to lung tissue, and studies have shown that tumors can increase the expression of this gene in a variety of cells.
- Exons 1-11 and 3‘UTR of the mouse RAGE gene that encodes the full-length protein was replaced by human RAGE exons 1-11 and 3’UTR in B-hRAGE mice plus.
- Mouse RAGE protein was only detectable in wild-type C57BL/6 mice. Human RAGE protein was detectable in homozygous B-hRAGE mice plus but not in wild-type mice.
- Mouse RAGE mRNA was only detectable in wild-type C57BL/6 mice. Human RAGE mRNA was detectable in homozygous B-hRAGE mice plus but not in wild-type mice.
- Application: This product is used for pharmacodynamics and safety evaluation of drugs for various chronic degenerative diseases and neoplastic diseases.
Targeting strategy
Gene targeting strategy for B-hRAGE mice plus. Exons 1-11 and 3‘UTR of the mouse RAGE gene that encodes the full-length protein was replaced by human RAGE exons 1-11 and 3’UTR in B-hRAGE mice plus.
mRNA expression analysis
Strain specific analysis of RAGE mRNA expression in wild-type C57BL/6 mice and B-hRAGE mice plus by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hRAGE mice plus (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human RAGE primers. Mouse RAGE mRNA was detectable only in wild-type C57BL/6 mice. Human RAGE mRNA was detectable only in homozygous B-hRAGE mice plus but not in wild-type mice.
Strain specific analysis of RAGE expression in wild-type C57BL/6N mice and heterozygous humanized B-hRAGE mice plus by RT-qPCR. Blood, and blood RNA were isolated from wild-type C57BL/6N mice (+/+) (n=3, 9-week-old) and B-hRAGE mice plus (n=5, 9-week-old), then cDNA libraries were synthesized by reverse transcription, followed by real-time quantitative PCR with RAGE primers. The mRNA levels of RAGE was in detectable in B-hRAGE mice plus with Applied Biosystems SYBR, but not wild-type C57BL/6N mice. The mRNA levels of RAGE in lung from B-hRAGE mice plus was higher than that in blood from B-hRAGE mice plus.
Western blot analysis of RAGE protein expression in homozygous B-hRAGE mice plus. Lung tissue lysates were collected from wild-type C57BL/6N mice (+/+), homozygous B-hRAGE mice plus (H/H) and B-Ager KO mice(RM), and then analyzed by western blot with species-specific anti-RAGE antibody. 40 μg total proteins were loaded for western blotting analysis. RAGE was detected in lung.
Protein expression analysis in serum
