B-hRAGE mice
| Strain Name |
C57BL/6N-Agertm1(AGER)Bcgen/Bcgen
|
Common Name | B-hRAGE mice |
| Background | C57BL/6N | Catalog number | 111107 |
|
Related Genes |
AGER (Advanced glycosylation end-product specific receptor), RAGE, SCARJ1 | ||
|
NCBI Gene ID |
11596 | ||
mRNA expression analysis
Strain specific analysis of RAGE gene expression in WT and B-hRAGE mice by RT-PCR. Mouse Rage mRNA was detectable in splenocytes of wild-type (+/+) mice. Human RAGE mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Western blot analysis of RAGE protein expression in homozygous B-hRAGE mice. Lung, spleen, and brain tissue lysates were collected from wild-type C57BL/6N mice (+/+), homozygous B-hRAGE mice (H/H), and then analyzed by western blot with species-specific anti-RAGE antibody(R&D, MAB11451). 40 μg total proteins were loaded for western blotting analysis. RAGE was detected in lung.
Protein expression analysis in serum
Strain specific RAGE expression analysis in wild-type C57BL/6N mice and homozygous humanized B-hRAGE mice by ELISA. Serum was collected from wild-type C57BL/6N mice (+/+) (male, n=3, 7-week-old) and heterozygous B-hRAGE mice (H/H) (male, n=3, 7-week-old). Expression level of mouse and human RAGE were analyzed by ELISA (Human RAGE Quantikine ELISA Kit: R&D, DRG00). Human RAGE was exclusively detectable in homozygous B-hRAGE mice(n=3). Values are expressed as mean ± SEM.
Strain specific RAGE expression analysis in wild-type C57BL/6N mice and homozygous humanized B-hRAGE mice by ELISA. Lung homogenates were collected from wild-type C57BL/6N mice (+/+) (male, n=3, 7-week-old) and heterozygous B-hRAGE mice (H/H) (male, n=3, 7-week-old). Expression level of mouse and human RAGE were analyzed by ELISA (Human RAGE Quantikine ELISA Kit: R&D, DRG00). Human RAGE was exclusively detectable in homozygous B-hRAGE mice(n=3). Values are expressed as mean ± SEM.
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