Strain specific analysis of SELE gene expression in wild-type C57BL/6 mice and B-hSELE/hSELL mice by RT-PCR. Mouse SELE mRNA was detectable only in bladder cells of wild-type C57BL/6 mice (+/+). Human SELE mRNA was detectable only in homozygous B-hSELE/hSELL mice (H/H; H/H), but not in wild-type mice. 

Strain specific analysis of SELL gene expression in wild-type C57BL/6 mice and B-hSELE/hSELL mice by RT-PCR. Mouse SELL mRNA was detectable only in splenocytes of wild-type C57BL/6 mice (+/+). Human SELL mRNA was detectable only in homozygous B-hSELE/hSELL mice (H/H; H/H), but not in wild-type mice. 

Protein expression analysis

Strain specific SELE expression analysis in homozygous B-hSELE/hSELL mice by ELISA. Serum were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSELE/hSELL mice (H/H; H/H), and analyzed by ELISA with species-specific SELE ELISA kit. Mouse SELE was detectable in wild-type mice. Human SELE was exclusively detectable in homozygous B-hSELE/hSELL mice (H/H; H/H) but not wild-type mice.

Strain specific SELL expression analysis in homozygous B-hSELE/hSELL mice by ELISA. Serum were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSELE/hSELL mice (H/H; H/H), and analyzed by ELISA with species-specific SELL ELISA kit. Mouse SELL was detectable in wild-type mice. Human SELL was exclusively detectable in homozygous B-hSELE/hSELL mice (H/H; H/H) but not wild-type mice.