B-hSIRPA/hCD47,Prkdc/Il2rg KO mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Humanizad Mouse Models

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B-hSIRPA/hCD47,Prkdc/Il2rg KO mice

Strain Name	C57BL/6-Sirpa^{tm1(SIRPA)Bcgen} Cd47^{tm1(CD47)Bcgen} Prkdc^tm1Bcgen Il2rg^tm1Bcgen/Bcgen	Common Name	B-hSIRPA/hCD47,Prkdc/Il2rg KO mice
Background	C57BL/6	Catalog number	140739
Aliases	SIRPA: BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP; CD47: IAP, MER6, OA3; Prkdc: scid; Il2rg: CD132, [g]c, gamm, gamma(, gamma(c), gc, p64
NCBI Gene ID	19261,16423,19090,16186

Protein expression analysis

Species specific SIRPα and CD47 expression analysis in B-hSIRPA/hCD47,Prkdc/Il2rg KO mice by flow cytometry. Splenocytes isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (H/H) were analyzed by flow cytometry with anti-SIRPα and anti-CD47 antibodies. Human SIRPα and CD47 were exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.

Protein expression analysis

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Species specific SIRPα and CD47 expression analysis in B-hSIRPA/hCD47,Prkdc/Il2rg KO mice by flow cytometry. Splenocytes were isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (H/H) stimulated with anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-SIRPα and anti-CD47 antibodies. Human SIRPα and CD47 were exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.

Analysis of leukocyte subpopulations in spleen

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Analysis of leukocyte subpopulations in spleen by FACS

Splenocytes were isolated from C57BL/6, B-NDG mice and B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (n=3, 11-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. Single live cells were gated for CD45 population and used for further analysis as indicated here. T, B and NK cells were undetectable in B-NDG mice and B-hSIRPA/hCD47,Prkdc/Il2rg KO mice.