B-hSIRPA/hCD47/hCD3E mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Humanizad Mouse Models

Text

B-hSIRPA/hCD47/hCD3E mice

Strain Name	C57BL/6-Sirpa^{tm1(SIRPA)Bcgen} Cd47^{tm1(CD47)Bcgen} Cd3e^{tm2(CD3E)Bcgen}/Bcgen	Common Name	B-hSIRPA/hCD47/hCD3E mice
Background	C57BL/6	Catalog number	131590
Aliases	CD3E, IMD18, T3E, TCRE, CD3e molecule; SIRPA, BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP, signal regulatory protein alpha; CD47, IAP, MER6, OA3, CD47 molecule
NCBI Gene ID	19261,6423,12501

Targeting strategy

Gene targeting strategy for B-hSIRPA/hCD47/hCD3E mice. The exons 2-6 of mouse Cd3e gene that encode the IgV domain were replaced by human CD3E exons 2-7 in B-hSIRPA/hCD47/hCD3E mice. The exon 2 of mouse Cd47 gene that encodes the extracellular domain was replaced by exon2 of human CD47 gene in B-hSIRPA/hCD47/hCD3E mice. The exon 2 of mouse Sirpα gene that encodes the extracellular domain was replaced by exon2 of human SIRPα in B-hSIRPA/hCD47/hCD3E mice.

Protein expression analysis

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Strain specific CD3E expression analysis in homozygous B-hSIRPA/hCD47/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E mice (H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).

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Strain specific CD47 expression analysis in homozygous B-hSIRPA/hCD47/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E mice (H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD47 antibody. Mouse CD47 was detectable in WT mice (+/+). Human CD47 was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E mice (H/H;H/H;H/H) but not in WT mice (+/+).

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Strain specific SIRPA expression analysis in homozygous B-hSIRPA/hCD47/hCD3E mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E mice (H/H;H/H;H/H), and analyzed by flow cytometry with anti-SIRPA antibody. SIRPA was detectable in WT mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E mice (H/H;H/H;H/H) due to the cross-reactivity of antibodies.