B-hTIM4 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6-Timd4^{tm1(TIMD4)Bcgen}/Bcgen	Common Name	B-hTIM4 mice
Background	C57BL/6	Catalog number	110837
Aliases	SMUCKLER, TIM4
NCBI Gene ID	276891

mRNA expression analysis

Strain specific analysis of TIM4 gene expression in wild-type C57BL/6 mice and B-hTIM4 mice by RT-PCR. Mouse TIM4 mRNA was detectable only in liver of wild-type C57BL/6 mice (+/+). Human TIM4 mRNA was detectable only in homozygous B-hTIM4 mice (H/H), but not in wild-type mice.

Protein expression analysis

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Strain specific TIM4 expression analysis in homozygous B-hTIM4 mice by flow cytometry. Macrophages in peritoneal lavage fluid were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTIM4 mice (H/H), and analyzed by flow cytometry with species-specific anti-TIM4 antibody. Mouse TIM4 was detectable in wild-type C57BL/6 mice. Human TIM4 was exclusively detectable in homozygous B-hTIM4 mice but not in wild-type C57BL/6 mice.