| Strain Name |
C57BL/6-Tnfrsf1btm1(TNFRSF1B)Bcgen Tnfrsf1atm1(TNFRSF1A)Bcgen/Bcgen
|
Common Name | B-hTNFR2/hTNFR1 mice |
| Background | C57BL/6 | Catalog number |
120802 |
|
Related Genes |
TNFRSF1B (Tumor necrosis factor receptor superfamily, member 1b); TNFRSF1A (Tumor necrosis factor receptor superfamily, member 1a) |
||
|
NCBI Gene ID |
21938,21937 | ||
mRNA expression analysis
Strain specific analysis of TNFR1 and TNFR2 gene expression in WT and B-hTNFR2/TNFR1 mice by RT-PCR. Mouse Tnfr1 and Tnfr2 mRNA were detectable only in splenocytes of wild-type (+/+) mice. Human TNFR1 and TNFR2 mRNA were detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Strain specific TNFR2 expression analysis in homozygous B-hTNFR2/hTNFR1 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2/hTNFR1 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2/hTNFR1 but not WT mice.
