Strain specific analysis of VSIR gene expression in WT and hVSIR mice by RT-PCR. 

Mouse vsir mRNA was detectable  in bone marrow cell of wild-type (+/+) mice and heterozygous (H/+) mice. Human VSIR mRNA was detectable only in H/+, but not in +/+ mice. 

Strain specific VSIR expression analysis in homozygous B-hVSIR mice by flow cytometry. 

Bone marrow and blood were collected from WT and homozygous B-hVSIR (H/H) mice in vivo, and analyzed by flow cytometry with species-specific anti-VSIR antibody. Mouse VSIR was detectable in WT mice. Human VSIR was exclusively detectable in homozygous B-hVSIR but not WT mice.