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B-hVSIR mice
Strain Name
C57BL/6N-VSIRtm2(Vsir)Bcgen/Bcgen
Common Name  B-hVSIR mice
Background C57BL/6N Catalog number  110049
Related Genes 
B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
NCBI Gene ID
74048

mRNA expression analysis


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Strain specific analysis of VSIR gene expression in WT and hVSIR mice by RT-PCR. 
Mouse vsir mRNA was detectable  in bone marrow cell of wild-type (+/+) mice and heterozygous (H/+) mice. Human VSIR mRNA was detectable only in H/+, but not in +/+ mice. 


Protein expression analysis


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Strain specific VSIR expression analysis in homozygous B-hVSIR mice by flow cytometry. 
Bone marrow and blood were collected from WT and homozygous B-hVSIR (H/H) mice in vivo, and analyzed by flow cytometry with species-specific anti-VSIR antibody. Mouse VSIR was detectable in WT mice. Human VSIR was exclusively detectable in homozygous B-hVSIR but not WT mice.