B-NDG hF3 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

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B-NDG hF3 mice

Strain Name	NOG.CB17-Prkdc^scidIl2rg^{tm1 Bcgen}F3^tm1(F3)Bcgen/Bcgen	Common Name	B-NDG hF3 mice
Background	B-NDG	Catalog number	113228
Related Genes	CD142, TF, TFA
NCBI Gene ID	2152

Description

CD142, also known as Tissue Factor (TF), Coagulation Factor III, and Thromboplastin, is a 45 kD type I transmembrane glycoprotein. It is expressed on the surface of a variety of cells that are physically separated from the circulating blood which include smooth muscle cells, fibroblasts, keratinocytes, glomerular epithelial cells (cytoplasmic inclusions), astrocytes, myocardium, liver stromal cells, pancreas cells, and is also expressed on activated monocytes and stimulated endothelial cells.
CD142 is a high-affinity receptor for coagulation factor VII and initiates the extrinsic pathway of blood coagulation. CD142 also plays an important role in a variety of diseases such as sepsis, atherosclerosis and cancer.

Targeting strategy

Gene targeting strategy for B-NDG hF3 mice. The exons 1-6 of mouse F3 gene that encode extracellular, transmembrane domain and cytoplasmic domain is replaced by human counterparts in B-NDG hF3 mice. The promoter, 5’UTR and 3’UTR region of the mouse gene are also retained. The chimeric F3 expression is driven by endogenous mouse F3 promoter, while mouse F3 gene transcription and translation will be disrupted.

Protein expression analysis in endotheliocytes of Lung

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Strain specific F3/CD142 expression analysis in homozygous B-NDG hF3 mice by flow cytometry. Lung was collected from wild-type B-NDG (+/+) and homozygous B-NDG hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-human F3/CD142 antibody and anti-mouse F3/CD142 antibody (R＆D, FAB3178P; eBioscience, 17-1429-42). Mouse F3/CD142 was only detectable in wild-type mice. Human F3/CD142 was exclusively detectable in homozygous B-NDG hF3 mice but not in wild-type mice.

Protein expression analysis in brain

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Strain specific F3/CD142 expression analysis in homozygous B-NDG hF3 mice by flow cytometry. Brain was collected from wild-type B-NDG mice (+/+) and homozygous B-NDG hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-human F3/CD142 antibody and anti-mouse F3/CD142 antibody (R＆D, FAB3178P; eBioscience, 17-1429-42). Mouse F3/CD142 was only detectable in wild-type mice. Human F3/CD142 was exclusively detectable in homozygous B-NDG hF3 mice but not in wild-type mice.