Strain Name
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C57BL/6N-Amigo2tm1(AMIGO2)Bcgen/Bcgen
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Common Name
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B-hAMIGO2 mice
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Background
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C57BL/6N
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Catalog number
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111658
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Aliases
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AMIGO2: ALI1, AMIGO-2, DEGA
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Protein expression analysis
Western blot analysis of AMIGO2 protein expression in homozygous B-hAMIGO2 mice. Various tissue lysates were collected from C57BL/6 wildtype (+/+) mice and homozygous B-hAMIGO2 (H/H) mice, and then analyzed by western blot with anti-AMIGO2 antibody. 40 μg total proteins were loaded for western blotting analysis. AMIGO2 was detected in lung, kidney, stomach and spleen.
mRNA expression analysis
Strain specific analysis of AMIGO2 mRNA expression in wild-type C57BL/6 mice and B-hAMIGO2 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hAMIGO2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human AMIGO2 primers. Mouse Amigo2 mRNA was detectable only in wild-type C57BL/6 mice. Human AMIGO2 mRNA was detectable only in homozygous B-hAMIGO2 mice but not in wild-type mice.