B-hANPEP mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Other Humanized Mice

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B-hANPEP mice

Strain Name	C57BL/6-Anpep^{tm1(ANPEP)Bcgen}/Bcgen	Common Name	B-hANPEP mice
Background	C57BL/6	Catalog number	112554
Aliases	Apn; AP-M; AP-N; Cd13; P150

Protein expression analysis

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Strain specific ANPEP expression analysis in wild-type C57BL/6 and homozygous B-hANPEP mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was detectable in DCs of wild-type mice. hANPEP was detectable in DCs of homozygous B-hANPEP mice.

Protein expression analysis

Protein expression analysis

Strain specific ANPEP expression analysis in wild-type C57BL/6 and homozygous B-hANPEP mice by flow cytometry. Blood were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was weakly detectable in monocytes/macrophages of wild-type mice. hANPEP was detectable in monocytes/macrophages of homozygous B-hANPEP mice. Peritoneal washes were collected from wild-type C57BL/6 (+/+) and homozygous B-hANPEP mice (H/H) and analyzed by flow cytometry with species-specific anti-ANPEP antibody. mANPEP was detectable in macrophages of wild-type mice. hANPEP was detectable in macrophages of homozygous B-hANPEP mice.

mRNA expression analysis

Strain specific analysis of ANPEP mRNA expression in wild-type C57BL/6 mice and B-hANPEP mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hANPEP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ANPEP primers. Mouse Anpep mRNA was detectable only in wild-type C57BL/6 mice. Human ANPEP mRNA was detectable only in homozygous B-hANPEP mice but not in wild-type mice.