Strain Name
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C57BL/6N-Aoc3tm1(AOC3)Bcgen/Bcgen
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Common Name
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B-hAOC3 mice
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Background
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C57BL/6N
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Catalog number
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111626
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Aliases
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AOC3: HAPO, SSAO, VAP1,VAP-1
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mRNA expression analysis
Strain specific analysis of AOC3 mRNA expression in wild-type C57BL/6N mice and B-hAOC3 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hAOC3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human AOC3 primers. Mouse Aoc3 mRNA was detectable only in wild-type C57BL/6N mice. Human AOC3 mRNA was detectable only in homozygous B-hAOC3 mice but not in wild-type mice.
Protein expression analysis
Western blot analysis of AOC3 protein expression in homozygous B-hAOC3 mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hAOC3 mice (H/H), and then analyzed by western blot with anti-AOC3 antibody. 40μg total proteins were loaded for western blotting analysis. AOC3 was detected in heart, spleen, kidney, lung and stomach of wild-type C57BL/6N mice and spleen, lung and stomach in homozygous B-hAOC3 mice.