Strain specific APOE expression analysis in homozygous B-hAPOE4 mice by ELISA. Serum collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hAPOE4 mice (H/H) were analyzed by ELISA with species-specific APOE ELISA kit. Mouse APOE was detectable in wild-type mice (A). Human APOE was exclusively detectable in homozygous B-hAPOE4 mice but not in wild-type mice (B).

Strain specific APOE expression analysis in homozygous B-hAPOE4 mice by ELISA. Brain collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hAPOE4 mice (H/H) were extracted and APOE was measured in the brain homogenates using species-specific APOE ELISA kit and then normalized to total protein levels. Mouse APOE was detectable in wild-type mice (A). Human APOE was exclusively detectable in homozygous B-hAPOE4 mice but not in wild-type mice (B).

Strain specific analysis of APOE4 mRNA expression in wild-type C57BL/6J mice and B-hAPOE4 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hAPOE4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOE primers. Mouse Apoe mRNA was detectable only in wild-type mice. Human APOE4 mRNA was detectable only in homozygous B-hAPOE4 mice but not in wild-type mice.  And the results were confirmed via Sanger sequencing.