top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hBMP9 mice
Strain Name
C57BL/6N-Gdf2tm1(GDF2)Bcgen/Bcgen
Common Name  B-hBMP9 mice
Background C57BL/6N Catalog number 112354
Related Genes 

BMP9, HHT5, BMP-9

NCBI Gene ID
2658
Description

  • BMP9 is a secreted protein and after binding to its receptors, SMAD family transcription factors were recruited and activated, leading to gene expression.
  • Homozygous mice expressed the human BMP9 and developed normally.
  • This model is useful in studying the role of BMP9 in liver fibrosis.

mRNA expression analysis


from clipboard

Strain specific analysis of BMP9 mRNA expression in wild-type C57BL/6N mice and homozygous B-hBMP9 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hBMP9 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human BMP9 primers. Mouse Bmp9 mRNA was detectable only in wild-type C57BL/6N mice. Human BMP9 mRNA was detectable only in homozygous B-hBMP9 mice but not in wild-type mice. 

Protein expression analysis

from clipboard

Western blot analysis of BMP9 protein expression in homozygous B-hBMP9 mice. Plasma and serum were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hBMP9 mice (H/H), (male, 7-week-old, n=2) and then analyzed by western blot with anti-BMP9 antibody (Abcam, ab207318). 20 μg total proteins were loaded for western blotting analysis. The antibody used showed cross-recognition for mouse and human BMP9.