Strain specific C3 expression analysis in homozygous B-hC3 mice by ELISA. Serum were collected from wild-type mice (+/+) and homozygous B-hC3 mice (H/H), and analyzed by ELISA with species-specific anti-C3 antibody. Mouse C3 was detectable in wild-type mice. Human C3 was detectable in homozygous B-hC3 mice.

Strain specific C3 expression analysis in homozygous B-hC3 mice by ELISA. Serum were collected from different weeks including 10, 12 and 14-week of homozygous B-hC3 mice (H/H), and analyzed by ELISA with species-specific anti-C3 antibody. Human C3 was detectable in homozygous B-hC3 mice. The expression of human C3 did not change significantly with the increase of age. (*p<0.05,***p<0.001, ****p<0.0001)

Analysis of blood biochemical in hC3 and WT. Serum were collected from wild-type mice (+/+) and homozygous B-hC3 mice (H/H), and analyzed for biochemistry. Biochemistry analysis revealed that the B-hC3 mice presented with significant elevation of BUN and sCysC(10w and 14w). The B-hC3 mice also showed significant elevation of liver injury markers, such as alanine aminotransferase(ALT), aspartate aminotransferase(AST), and alkaline Phosphatase(ALP).(*p<0.05,***p<0.001, ****p<0.0001)