Strain Name
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C57BL/6N-Car9tm1(CA9)Bcgen/Bcgen
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Common Name
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B-hCAIX mice
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Background
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C57BL/6
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Catalog number
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110855
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Aliases
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CA9, CAIX, MN, carbonic anhydrase 9
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mRNA expression analysis
Strain specific analysis of CAIX gene expression in wild type (WT) mice and B-hCAIX mice by RT-PCR. Mouse Car9 mRNA was detectable only in stomach of WT mice (+/+). Human CAIX mRNA was detectable only in homozygous B-hCAIX mice (H/H) but not in WT mice (+/+).
IHC analysis of CAIX expression
Immunohistochemical (IHC) analysis of CAIX expression in homozygous B-hCAIX mice. The stomach and liver were collected from WT and homozygous B-hCAIX mice (H/H) and analyzed by IHC with anti-human CAIX antibody. Human CAIX was exclusively detectable in homozygous B-hCAIX mice (H/H) but not in WT mice (+/+). The arrow indicates tissue cells with positive CAIX staining.