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B-hCD105 mice
Strain Name C57BL/6-Engtm1(ENG)Bcgen/Bcgen Common Name  B-hCD105 mice
Background C57BL/6 Catalog number 110886
Aliases 
ENG: END, HHT1, ORW1
Protein expression analysis

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Western blot analysis of CD105 protein expression in homozygous B-hCD105 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD105 mice (H/H), and then analyzed by western blot with species-specific anti-CD105 antibody. 20 μg total proteins were loaded for western blotting analysis. hCD105 was detected in lung,heart,spleen,kidney,liver and skin from homozygous mice.

mRNA expression analysis

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Strain specific analysis of CD105 mRNA expression in wild-type C57BL/6 mice and B-hCD105 mice by RT-PCR. Lung RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCD105 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CD105 primers. Mouse CD105 mRNA was detectable only in wild-type C57BL/6 mice. Human CD105 mRNA was detectable only in homozygous B-hCD105 mice but not in wild-type mice.