Strain specific analysis of CD1D gene expression in wild-type C57BL/6 mice and hCD1D mice by RT-PCR. Mouse Cd1d mRNA was detectable only in splenocytes of wild-type C57BL/6 mice (+/+). Human CD1D mRNA was detectable only in homozygous B-hCD1D mice (H/H), but not in wild-type mice. 

Strain specific CD1D expression analysis in homozygous B-hCD1D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD1D mice (H/H), and analyzed by flow cytometry with species-specific anti-CD1D antibody. Mouse CD1D was detectable in wild-type mice. Human CD1D was exclusively detectable in homozygous B-hCD1D but not in wild-type mice.