Strain Name
|
C57BL/6N-Cd300atm1(CD300A)Bcgen/Bcgen
|
Common Name
|
B-hCD300A mice
|
Background
|
C57BL/6N
|
Catalog number
|
111428
|
Aliases
|
CD300A:CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9,
CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60
|
mRNA expression analysis
Strain specific analysis of CD300A gene expression in wild type C57BL/6 mice and homozygous B-hCD300A mice by RT-PCR. Splenocytes were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H). Mouse CD300a mRNA was detectable only in wild type C57BL/6 mice. Human CD300A mRNA was detectable only in homozygous B-hCD300A mice, but not in wild type C57BL/6 mice.
Protein expression analysis
Strain specific CD300A expression analysis in homozygous B-hCD300A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H), and analyzed by flow cytometry with anti-hCD300A antibody. Human CD300A was detectable in B cells, NK cells and DCs in homozygous B-hCD300A mice (H/H).
Protein expression analysis
Strain specific CD300A expression analysis in homozygous B-hCD300A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H), and analyzed by flow cytometry with anti-hCD300A antibody. Human CD300A was detectable in B cells, NK cells, DCs, neutrophils, monocytes and macrophages in homozygous B-hCD300A mice (H/H).