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B-hCD98HC mice
Strain Name 

C57BL/6N-Slc3a2tm1(SLC3A2)Bcgen/Bcgen  

Common Name 

B-hCD98HC mice

Background C57BL/6N Catalog number

110983

Aliases 

SLC3A2, 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, CD98 heavy chain


mRNA expression analysis



Strain specific analysis of CD98 gene expression in wild type (WT) mice and B-hCD98HC mice by RT-PCR.

Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).


Protein expression analysis





Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC mice (H/H), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).


Protein expression analysis in brain


from clipboard


Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by Immunofluorescence staining. Brain was collected from wild type (WT) mice and homozygous B-hCD98HC mice (female,8 week old) and processed into paraffin sections. The paraffin sections were stained using an species-specific anti-human CD98HC antibody (green). Brain tissues were co-stained with an anti-mouse CD31 antibody to visualize microvascular endothelial cells (red). Representative results of WT mice (A) and homozygous B-hCD98HC mice (B) are shown. The results indicated that human CD98 was exclusively detectable on brain microvascular endothelium of homozygous B-hCD98HC mice, but not in WT mice.