Strain Name |
C57BL/6N-Slc3a2tm1(SLC3A2)Bcgen/Bcgen
|
Common Name |
B-hCD98HC mice |
Background | C57BL/6N | Catalog number |
110983 |
Aliases |
SLC3A2, 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, CD98 heavy chain |
Strain specific analysis of CD98 gene expression in wild type (WT) mice and B-hCD98HC mice by RT-PCR.
Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC mice (H/H), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
Protein expression analysis in brain
Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by Immunofluorescence staining. Brain was collected from wild type (WT) mice and homozygous B-hCD98HC mice (female,8 week old) and processed into paraffin sections. The paraffin sections were stained using an species-specific anti-human CD98HC antibody (green). Brain tissues were co-stained with an anti-mouse CD31 antibody to visualize microvascular endothelial cells (red). Representative results of WT mice (A) and homozygous B-hCD98HC mice (B) are shown. The results indicated that human CD98 was exclusively detectable on brain microvascular endothelium of homozygous B-hCD98HC mice, but not in WT mice.