B-hCDH17 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

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B-hCDH17 mice

Strain Name	C57BL/6-Cdh17^{tm1(CDH17)Bcgen}/Bcgen	Common Name	B-hCDH17 mice
Background	C57BL/6	Catalog number	111593
Related Genes	HPT1; CDH16; HPT-1

mRNA expression analysis

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Strain specific analysis of CDH17 mRNA expression in wild-type C57BL/6 mice and B-hCDH17 mice by RT-PCR. Liver, colon and small intestine RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCDH17 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CDH17 primers. Human CDH17 mRNA was detectable in colon and small intestine of homozygous B-hCDH17 mice, not in liver. Meanwhile, the mouse Cdh17 mRNA was detectable in colon and small intestine of wild-type C57BL/6 mice, not in liver.

Protein expression analysis

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Simple Western analysis of CDH17 protein expression in homozygous B-hCDH17 mice. Liver, colon and small intestine lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hCDH17 mice (H/H), and then analyzed by Simple Western with species-specific anti-hCDH17 antibody(abcam, ab109190). 3ug total proteins were loaded for simple western analysis. Human CDH17 protein was detected in the colon and small intestine of homozygous B-hCDH17 mice, whereas human CDH17 protein was not detected in the liver of homozygous B-hCDH17 mice.

CDH17 expression pattern analysis by IHC

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Representative human CDH17 expression in different tissues of B-hCDH17 mice by IHC. Tissues were stained with antibodies specific for human CDH17 (A-B, D-L) or anti-IgG antibody (C). Colon and ileum of B-hCDH17 mice show human CDH17 positive. Heart, liver, spleen, lung, kidney, stomach, and pancreas of B-hCDH17 mice show human CDH17 negative. Human gastric carcinoma cell line SNU-5 as a positive control (A); Colon tissues from the wild-type C57BL/6 mice as a negative control (B); Other tissues were got from homozygous B-hCDH17 mice (C-L). Original magnification ×200. Abbreviations: IHC, immunohistochemistry. The antibodies were provided by the client.