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B-hCSPG5 mice
Strain Name C57BL/6-Cspg5tm2(CSPG5)Bcgen/Bcgen Common Name  B-hCSPG5 mice
Background C57BL/6 Catalog number 112235
Aliases 

CSPG5: NGC

Protein expression analysis

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Western blot analysis of CSPG5 protein expression in homozygous B-hCSPG5 mice. Cortex lysates were collected from C57BL/6 wild-type (+/+) mice and homozygous B-hCSPG5 mice (H/H), and then analyzed by western blot with species-specific anti-CSPG5 antibody. 40 μg total proteins were loaded for western blotting analysis. CSPG5 protein was detected in the cortex of wild-type C57BL/6 mice and homozygous B-hCSPG5 mice, as the antibody is cross-recognize both human and mouse CSPG5. It is impossible to conclude from the data that the observed three bands are CSPG5 three isoforms.

mRNA expression analysis

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Strain specific analysis of CSPG5 mRNA expression in wild-type C57BL/6 mice and B-hCSPG5 mice by RT-PCR. Cortex RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCSPG5 mice(H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CSPG5 primers. Mouse Cspg5 mRNA was detectable only in wild-type C57BL/6 mice. Human CSPG5 mRNA was detectable only in homozygous B-hCSPG5 mice but not in wild-type mice.