Strain specific analysis of EGFR gene expression in wild type (WT) mice and B-hEGFR/hMET mice by RT-PCR. Mouse Egfr mRNA was detectable only in liver and kidney of WT mice (EGFR+/+MET+/+). Human EGFR mRNA was detectable only in B-hEGFR/hMET mice (EGFRH/HMETH/+) but not in WT mice (EGFR+/+MET+/+). The positive band was confirmed to be correct by sequencing.

Strain specific analysis of MET gene expression in wild type (WT) mice and B-hEGFR/hMET mice by RT-PCR. Mouse Met mRNA was detectable in liver and kidney of WT mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+). Human MET mRNA was detectable only in B-hEGFR/hMET mice (EGFRH/HMETH/+) but not in WT mice (EGFR+/+MET+/+). The positive band was confirmed to be correct by sequencing.

Strain specific MET expression analysis in homozygous B-hEGFR/hMET mice by western blot. Liver and kidney were collected from wild type (WT) mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+), and analyzed by western blot with anti-MET antibody. MET was detectable in WT mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+) due to the cross-reactivity of antibodies.

Immunohistochemical (IHC) analysis of EGFR expression in homozygous B-hEGFR/hMET mice. The liver and kidney were collected from wild type (WT) mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+) and analyzed by IHC with anti-EGFR antibody. EGFR was detectable in WT mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+) due to the cross-reactivity of antibodies.