B-hFXII mice
| Strain Name |
C57BL/6N-F12tm1(F12)Bcgen/Bcgen
|
Common Name | B-hFXII mice |
| Background | C57BL/6N | Catalog number |
112750 |
| Aliases |
HAE3, HAEX, HAF |
||
|
NCBI Gene ID |
2161 | ||
- FXII is a protease that is primarily produced in the liver and circulates as a single-chain zymogen in plasma, playing an important role in the intrinsic pathway of blood coagulation and hemostasis. Upon contact with negatively charged surfaces, FXII converts into its double-stranded active form, FXIIa. The absence of FXII in humans and mice does not affect normal hemostasis ability, and the lack of FXII can inhibit thrombus formation, making it an important target in thrombosis treatment.
- Application: This product is used for pharmacodynamics and safety evaluation of cardiovascular diseases such as thrombosis.
mRNA expression analysis in humanized B-hFXII mice
Strain specific analysis of FXII mRNA expression in wild-type C57BL/6N mice and B-hFXII mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6N mice (+/+) and heterozygous B-hFXII mice (H/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FXII primers. Mouse FXII mRNA were detectable in wild-type C57BL/6N and heterozygous B-hFXII mice. Human FXII mRNA was only detectable in heterozygous B-hFXII mice.
Protein expression analysis in humanized B-hFXII mice
Strain specific FXII expression analysis in homozygous B-hFXII mice by ELISA. Serum and plasma were collected from wild-type mice C57BL/6N mice (+/+) and homozygous B-hFXII mice (H/H) (n=3), and analyzed by ELISA with species-specific FXII ELISA kit. Mouse FXII was only detectable in wild-type mice (A). Human FXII was exclusively detectable in homozygous mice but not in wild-type mice (B).
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