B-hGPC1 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6N-Gpc1^tm1(GPC1)/Bcgen	Common Name	B-hGPC1 mice
Background	C57BL/6N	Catalog number	111239
Aliases	GPC1, glypican, glypican 1

mRNA expression analysis

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Strain specific analysis of GPC1 gene expression in wild-type mice and B-hGPC1 mice by RT-PCR. Mouse Gpc1 mRNA was detectable only in lung of wild-type mice (+/+). Human GPC1 mRNA was detectable only in homozygous B-hGPC1 mice (H/H) but not in wild-type mice (+/+).

Protein expression analysis

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Strain specific GPC1 expression analysis in homozygous B-hGPC1 mice by western blot. Heart was collected from wild-type mice (+/+) and homozygous B-hGPC1 mice (H/H), and analyzed by western blot with anti-GPC1 antibody. Human GPC1 was detectable in homozygous B-hGPC1 mice (H/H).