Strain Name
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C57BL/6-Hamptm1(HAMP)Bcgen/Bcgen
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Common Name
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B-hHAMP mice
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Background
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C57BL/6N
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Catalog number
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111019
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Aliases
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HAMP: HEPC, HFE2B, LEAP1, PLTR
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Protein expression analysis
Strain specific HAMP expression analysis in homozygous B-hHAMP mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hHAMP mice (H/H), and analyzed by ELISA with species-specific HAMP ELISA kit. Human HAMP was exclusively detectable in homozygous B-hHAMP mice but not in wild-type mice.
mRNA expression analysis
Strain specific analysis of HAMP mRNA expression in wild-type C57BL/6 mice and B-hHAMP mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hHAMP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human HAMP primers. Mouse Hamp mRNA were detectable in wild-type C57BL/6. Human HAMP mRNA was detectable in homozygous B-hHAMP mice.