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B-hIBSP mice
Strain Name C57BL/6N-Ibsptm1(IBSP)Bcgen/Bcgen Common Name  B-hIBSP mice
Background C57BL/6N Catalog number 110930
Aliases 

IBSP: BSP, BNSP, SP-II, BSP-II

Protein expression analysis

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Strain specific IBSP expression analysis in homozygous B-hIBSP mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H) and analyzed by ELISA with IBSP ELISA kit. Mouse IBSP was detectable in wild-type mice and homozygous B-hIBSP mice as the kit has partly cross reactions with human and mouse (A). Human IBSP was exclusively detectable in homozygous B-hIBSP mice but not in wild-type mice (B).

mRNA expression analysis

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Strain specific analysis of IBSP mRNA expression in wild-type C57BL/6 mice and B-hIBSP mice by RT-PCR. Bone marrow RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human IBSP primers. Mouse Ibsp mRNA was detectable only in wild-type C57BL/6 mice. Human IBSP mRNA was detectable only in homozygous B-hIBSP mice but not in wild-type mice.