Strain Name
|
C57BL/6N-Ibsptm1(IBSP)Bcgen/Bcgen
|
Common Name
|
B-hIBSP mice
|
Background
|
C57BL/6N
|
Catalog number
|
110930
|
Aliases
|
IBSP: BSP, BNSP, SP-II, BSP-II
|
Protein expression analysis
Strain specific IBSP expression analysis in homozygous B-hIBSP mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H) and analyzed by ELISA with IBSP ELISA kit. Mouse IBSP was detectable in wild-type mice and homozygous B-hIBSP mice as the kit has partly cross reactions with human and mouse (A). Human IBSP was exclusively detectable in homozygous B-hIBSP mice but not in wild-type mice (B).
mRNA expression analysis
Strain specific analysis of IBSP mRNA expression in wild-type C57BL/6 mice and B-hIBSP mice by RT-PCR. Bone marrow RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human IBSP primers. Mouse Ibsp mRNA was detectable only in wild-type C57BL/6 mice. Human IBSP mRNA was detectable only in homozygous B-hIBSP mice but not in wild-type mice.