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B-hITGA7 mice
Strain Name
C57BL/6-Itga7tm1(ITGA7)Bcgen/Bcgen
Common Name  B-hITGA7 mice
Background C57BL/6 Catalog number 112502
Aliases 
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Protein expression analysis

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Western blot analysis of ITGA7 protein expression in homozygous B-hITGA7 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA7 mice (H/H), and then analyzed by western blot with anti-ITGA7 antibody(thermofisher PA5-37435, cross-reactive between human and mouse). 40 μg total proteins were loaded for western blotting analysis. ITGA7 was detected in stomach and lung.

mRNA expression analysis

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Strain specific analysis of ITGA7 mRNA expression in wild-type C57BL/6 mice and B-hITGA7 mice by RT-PCR. Stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA7 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ITGA7 primers. Mouse Itga7 mRNA was detectable only in wild-type C57BL/6 mice. Human ITGA7 mRNA was detectable only in homozygous B-hITGA7 mice but not in wild-type mice.