top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hJAG1 mice
Strain Name C57BL/6-Jag1tm1(JAG1)Bcgen/Bcgen Common Name  B-hJAG1 mice
Background C57BL/6 Catalog number 110997
Aliases 

AGS, AHD, AWS, HJ1, AGS1, DCHE, CD339, JAGL1, CMT2HH

Protein expression analysis

from clipboard


Western blot analysis of JAG1 protein expression in homozygous B-hJAG1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hJAG1 mice (H/H), and then analyzed by western blot with anti-JAG1 antibody. 72 μg total proteins were loaded for western blotting analysis. JAG1 protein was detected in the kidney, lung, heart, spleen of wild-type C57BL/6 mice and homozygous B-hJAG1 mice, as the antibody is cross-recognize both human and mouse JAG1.

mRNA expression analysis

from clipboard


Strain specific analysis of JAG1 mRNA expression in wild-type C57BL/6 mice and B-hJAG1 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hJAG1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human JAG1 primers. Mouse Jag1 mRNA was detected in wild-type C57BL/6 mice. Human JAG1 mRNA was detected in homozygous B-hJAG1 mice but not in wild-type mice.