Strain Name
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C57BL/6N-Jam3tm1(JAM3)Bcgen/Bcgen
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Common Name
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B-hJAM3 mice
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Background
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C57BL/6N
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Catalog number
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111256
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Aliases
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JAM3, JAM-2, JAM-3, JAM-C, JAMC, junctional adhesion molecule 3
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mRNA expression analysis
Strain specific analysis of JAM3 gene expression in wild type (WT) mice and B-hJAM3 mice by RT-PCR. Mouse Jam3 mRNA was detectable only in lung of WT mice (+/+). Human JAM3 mRNA was detectable only in homozygous B-hJAM3 mice (H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific JAM3 expression analysis in homozygous B-hJAM3 mice by flow cytometry. Hematopoietic stem cells (HSCs) from bone marrow were collected from wild type (WT) mice (+/+) and homozygous B-hJAM3 mice (H/H), and analyzed by flow cytometry with species-specific anti-JAM3 antibody. Human JAM3 was exclusively detectable in homozygous B-hJAM3 mice (H/H) but not in WT mice.
Strain specific JAM3 expression analysis in homozygous B-hJAM3 mice by flow cytometry. Endothelial cells from lung were collected from wild type (WT) mice (+/+) and homozygous B-hJAM3 mice (H/H), and analyzed by flow cytometry with species-specific anti-JAM3 antibody. Human JAM3 was exclusively detectable in homozygous B-hJAM3 mice (H/H) but not in WT mice (+/+).