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B-hLSR mice
Strain Name C57BL/6-Lsrtm1(LSR)Bcgen/Bcgen Common Name  B-hLSR mice
Background C57BL/6 Catalog number 112957
Aliases 

LSR: ILDR3; LISCH7

Protein expression analysis

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Western blot analysis of LSR protein expression in homozygous B-hLSR mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hLSR mice (H/H), and then analyzed by western blot with anti-LSR antibody. 40 μg total proteins were loaded for western blotting analysis. 

mRNA expression analysis

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Strain specific analysis of LSR mRNA expression in wild-type C57BL/6 mice and B-hLSR mice by RT-PCR. Small intestine RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLSR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LSR primers. Mouse Lsr mRNA was detectable only in wild-type mice. Human LSR mRNA was detectable only in homozygous B-hLSR mice but not in wild-type mice.