Strain Name
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C57BL/6-Lsrtm1(LSR)Bcgen/Bcgen
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Common Name
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B-hLSR mice
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Background
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C57BL/6
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Catalog number
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112957
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Aliases
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LSR: ILDR3; LISCH7
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Protein expression analysis
Western blot analysis of LSR protein expression in homozygous B-hLSR mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hLSR mice (H/H), and then analyzed by western blot with anti-LSR antibody. 40 μg total proteins were loaded for western blotting analysis.
mRNA expression analysis
Strain specific analysis of LSR mRNA expression in wild-type C57BL/6 mice and B-hLSR mice by RT-PCR. Small intestine RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLSR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LSR primers. Mouse Lsr mRNA was detectable only in wild-type mice. Human LSR mRNA was detectable only in homozygous B-hLSR mice but not in wild-type mice.