B-hMST1R mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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동물/세포 모델

동물/세포모델

Humanized Mouse Models

Other Humanized Mice

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B-hMST1R mice

Strain Name	C57BL/6-Mst1r^{tm1(MST1R)Bcgen}/Bcgen	Common Name	B-hMST1R mice
Background	C57BL/6N	Catalog number	112535
Aliases	MST1R: CD136, CDw136, NPCA3, PTK8, RON, SEA, p185-Ron

mRNA expression analysis

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Strain specific analysis of MST1R mRNA expression in wild-type C57BL/6 mice and B-hMST1R mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hMST1R mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human MST1R primers. Mouse Mst1r mRNA were detectable in wild-type C57BL/6. Human MST1R mRNA was detectable in homozygous B-hMST1R mice.

Protein expression analysis

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Western blot analysis of MST1R protein expression in homozygous B-hMST1R mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMST1R mice (H/H), and then analyzed by western blot with anti-MST1R antibody. 1 μg/μL total proteins were loaded for western blotting analysis by JESS. Human MST1R was exclusively detected in spleen, stomach, colon, lung, liver, heart and bladder in homozygous B-hMST1R mice.