B-hNPR1 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

Strain Name	C57BL/6-Npr1^{tm1(NPR1)Bcgen}/Bcgen	Common Name	B-hNPR1 mice
Background	C57BL/6	Catalog number	111347
Aliases	NPR1 (ANPa; NPRA; ANPRA; GUC2A; GUCY2A)

mRNA expression analysis

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Strain specific analysis of NPR1 gene expression in wild-type C57BL/6 mice and B-hNPR1 mice by RT-PCR. Mouse Npr1 mRNA was detectable only in ovary of wild-type C57BL/6 mice (+/+). Human NPR1 mRNA was detectable only in homozygous B-hNPR1 mice (H/H), but not in wild-type mice.

Protein expression analysis

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Strain specific NPR1 expression analysis in homozygous B-hNPR1 mice by western blot. Heart, liver, spleen, lung, brain and ovary were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNPR1 mice (H/H), and analyzed by western blot with a known cross-reactive anti-NPR1 antibody. The NPR1 protein can be detected in wild-type C57BL/6 mice and homozygous B-hNPR1 mice.