Strain Name
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C57BL/6-P2rx3tm1(P2RX3)Bcgen/Bcgen
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Common Name
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B-hP2RX3 mice
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Background
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C57BL/6
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Catalog number
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110776
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Aliases
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P2RX3: P2X3
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Protein expression analysis
Western blot analysis of P2RX3 protein expression in homozygous B-hP2RX3 mice. Dorsal root ganglia were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hP2RX3 mice (H/H), and then analyzed by western blot with anti-P2RX3 antibody. 30 μg total proteins were loaded for western blotting analysis. P2RX3 was detected in Dorsal root ganglia. Anti-P2RX3 antibody was cross reactive between human and mouse.
mRNA expression analysis
Strain specific analysis of P2RX3 mRNA expression in wild-type C57BL/6 mice and B-hP2RX3 mice by RT-PCR. Dorsal root ganglia RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hP2RX3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human P2RX3 primers. Mouse P2rx3 mRNA was detectable only in wild-type C57BL/6 mice. Human P2RX3 mRNA was detectable only in homozygous B-hP2RX3 mice but not in wild-type mice.